Part:BBa_K2491030:Experience

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Applications of BBa_K2491030

Clone verification. The following primers annealing in the TRFA region of the RK2 backbone vector and the kanamycin region were used to verify the construct by amplification and sequencing of the backbone: TRFA_F: 5’-CCGACGGATGTTCGACTATTT-3’, TRFA_R: 5’-AGTAAAGCGCTGGCTGAA-3’, KM_F: 5’-AAAGCCGTTTCTGTAATGAAGG-3’, and KM_R: 5’-CCTGTTGAACAAGTCTGGAAAG-3’. The data showed that the 2 regions were present. In addition, cultures containing the RK2 backbone harboring the RFP gene [biobrick part BBa_K2491030 containing biobrick part BBa_J04450] showed a red pellet (right vial) compared to a control (left vial) without the RFP gene.

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User Reviews

UNIQ7e10d01aed51461b-partinfo-00000000-QINU UNIQ7e10d01aed51461b-partinfo-00000001-QINU